Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate.
Identifieur interne : 000832 ( Main/Exploration ); précédent : 000831; suivant : 000833Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate.
Auteurs : Ahmed Abbas [États-Unis] ; Hasan Koc ; Feng Liu ; Ming TienSource :
- Current genetics [ 0172-8083 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Phanerochaete.
- métabolisme : Cellulose, Polyosides.
- physiologie : Phanerochaete.
- Bases de données génétiques, Bois, Cartographie peptidique, Protéomique, Spectrométrie de masse MALDI, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Cellulose (metabolism), Databases, Genetic (MeSH), Electrophoresis, Gel, Two-Dimensional (MeSH), Peptide Mapping (MeSH), Phanerochaete (genetics), Phanerochaete (physiology), Polysaccharides (metabolism), Proteomics (MeSH), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Wood (MeSH).
- MESH :
- chemical , metabolism : Cellulose, Polysaccharides.
- genetics : Phanerochaete.
- physiology : Phanerochaete.
- Databases, Genetic, Electrophoresis, Gel, Two-Dimensional, Peptide Mapping, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Wood.
Abstract
Two-dimensional (2-D) gel electrophoresis was used to separate the extracellular proteins produced by the white-rot fungus Phanerochaete chrysosporium. Solid-substrate cultures grown on red oak wood chips yielded extracellular protein preparations which were not suitable for 2-D gel analysis. However, pre-washing the wood chips with water helped decrease the amount of brown material which caused smearing on the acidic side of the isoelectric focusing gel. The 2-D gels from these wood-grown cultures revealed more than 45 protein spots. These spots were subjected to in-gel digestion with trypsin followed by either peptide fingerprint analysis by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) or by liquid chromatography (LC)/MS/MS sequencing. Data from both methods were analyzed by Protein Prospector and the local P. chrysosporium annotated database. MALDI-TOF/MS only identified two proteins out of 25 analyzed. This was most likely due to problems associated with glycosylation. Protein sequencing by LC/MS/MS of the same 25 proteins resulted in identification of 16 proteins. Most of the proteins identified act on either cellulose or hemicellulose or their hydrolysis products. Thus far no lignin peroxidase, Mn peroxidase or laccases have been detected.
DOI: 10.1007/s00294-004-0550-4
PubMed: 15551134
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate.</title><author><name sortKey="Abbas, Ahmed" sort="Abbas, Ahmed" uniqKey="Abbas A" first="Ahmed" last="Abbas">Ahmed Abbas</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Koc, Hasan" sort="Koc, Hasan" uniqKey="Koc H" first="Hasan" last="Koc">Hasan Koc</name></author><author><name sortKey="Liu, Feng" sort="Liu, Feng" uniqKey="Liu F" first="Feng" last="Liu">Feng Liu</name></author><author><name sortKey="Tien, Ming" sort="Tien, Ming" uniqKey="Tien M" first="Ming" last="Tien">Ming Tien</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15551134</idno><idno type="pmid">15551134</idno><idno type="doi">10.1007/s00294-004-0550-4</idno><idno type="wicri:Area/Main/Corpus">000872</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000872</idno><idno type="wicri:Area/Main/Curation">000872</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000872</idno><idno type="wicri:Area/Main/Exploration">000872</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate.</title><author><name sortKey="Abbas, Ahmed" sort="Abbas, Ahmed" uniqKey="Abbas A" first="Ahmed" last="Abbas">Ahmed Abbas</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Koc, Hasan" sort="Koc, Hasan" uniqKey="Koc H" first="Hasan" last="Koc">Hasan Koc</name></author><author><name sortKey="Liu, Feng" sort="Liu, Feng" uniqKey="Liu F" first="Feng" last="Liu">Feng Liu</name></author><author><name sortKey="Tien, Ming" sort="Tien, Ming" uniqKey="Tien M" first="Ming" last="Tien">Ming Tien</name></author></analytic><series><title level="j">Current genetics</title><idno type="ISSN">0172-8083</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulose (metabolism)</term><term>Databases, Genetic (MeSH)</term><term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term><term>Peptide Mapping (MeSH)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (physiology)</term><term>Polysaccharides (metabolism)</term><term>Proteomics (MeSH)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term><term>Wood (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bases de données génétiques (MeSH)</term><term>Bois (MeSH)</term><term>Cartographie peptidique (MeSH)</term><term>Cellulose (métabolisme)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (physiologie)</term><term>Polyosides (métabolisme)</term><term>Protéomique (MeSH)</term><term>Spectrométrie de masse MALDI (MeSH)</term><term>Électrophorèse bidimensionnelle sur gel (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulose</term><term>Polyosides</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Databases, Genetic</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Peptide Mapping</term><term>Proteomics</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bases de données génétiques</term><term>Bois</term><term>Cartographie peptidique</term><term>Protéomique</term><term>Spectrométrie de masse MALDI</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two-dimensional (2-D) gel electrophoresis was used to separate the extracellular proteins produced by the white-rot fungus Phanerochaete chrysosporium. Solid-substrate cultures grown on red oak wood chips yielded extracellular protein preparations which were not suitable for 2-D gel analysis. However, pre-washing the wood chips with water helped decrease the amount of brown material which caused smearing on the acidic side of the isoelectric focusing gel. The 2-D gels from these wood-grown cultures revealed more than 45 protein spots. These spots were subjected to in-gel digestion with trypsin followed by either peptide fingerprint analysis by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) or by liquid chromatography (LC)/MS/MS sequencing. Data from both methods were analyzed by Protein Prospector and the local P. chrysosporium annotated database. MALDI-TOF/MS only identified two proteins out of 25 analyzed. This was most likely due to problems associated with glycosylation. Protein sequencing by LC/MS/MS of the same 25 proteins resulted in identification of 16 proteins. Most of the proteins identified act on either cellulose or hemicellulose or their hydrolysis products. Thus far no lignin peroxidase, Mn peroxidase or laccases have been detected.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15551134</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0172-8083</ISSN><JournalIssue CitedMedium="Print"><Volume>47</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Current genetics</Title><ISOAbbreviation>Curr Genet</ISOAbbreviation></Journal><ArticleTitle>Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate.</ArticleTitle><Pagination><MedlinePgn>49-56</MedlinePgn></Pagination><Abstract><AbstractText>Two-dimensional (2-D) gel electrophoresis was used to separate the extracellular proteins produced by the white-rot fungus Phanerochaete chrysosporium. Solid-substrate cultures grown on red oak wood chips yielded extracellular protein preparations which were not suitable for 2-D gel analysis. However, pre-washing the wood chips with water helped decrease the amount of brown material which caused smearing on the acidic side of the isoelectric focusing gel. The 2-D gels from these wood-grown cultures revealed more than 45 protein spots. These spots were subjected to in-gel digestion with trypsin followed by either peptide fingerprint analysis by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) or by liquid chromatography (LC)/MS/MS sequencing. Data from both methods were analyzed by Protein Prospector and the local P. chrysosporium annotated database. MALDI-TOF/MS only identified two proteins out of 25 analyzed. This was most likely due to problems associated with glycosylation. Protein sequencing by LC/MS/MS of the same 25 proteins resulted in identification of 16 proteins. Most of the proteins identified act on either cellulose or hemicellulose or their hydrolysis products. Thus far no lignin peroxidase, Mn peroxidase or laccases have been detected.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Abbas</LastName><ForeName>Ahmed</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koc</LastName><ForeName>Hasan</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Feng</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Tien</LastName><ForeName>Ming</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2004</Year><Month>11</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Curr Genet</MedlineTA><NlmUniqueID>8004904</NlmUniqueID><ISSNLinking>0172-8083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>8024-50-8</RegistryNumber><NameOfSubstance UI="C007916">hemicellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030541" MajorTopicYN="N">Databases, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010449" MajorTopicYN="Y">Peptide Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>07</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>10</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2004</Year><Month>10</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>11</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>5</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>11</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15551134</ArticleId><ArticleId IdType="doi">10.1007/s00294-004-0550-4</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Chromatogr A. 2004 Jan 16;1023(2):317-20</Citation><ArticleIdList><ArticleId IdType="pubmed">14753699</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1993 Dec;59(12):4017-23</Citation><ArticleIdList><ArticleId IdType="pubmed">8285705</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1969 Feb;129(2):689-95</Citation><ArticleIdList><ArticleId IdType="pubmed">5812971</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1993 Oct;59(10):3492-4</Citation><ArticleIdList><ArticleId IdType="pubmed">8250570</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2004 Jun;22(6):695-700</Citation><ArticleIdList><ArticleId IdType="pubmed">15122302</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1996 Apr;62(4):1329-35</Citation><ArticleIdList><ArticleId IdType="pubmed">8919793</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1992 Nov;58(11):3466-71</Citation><ArticleIdList><ArticleId IdType="pubmed">16348798</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 1991 Jul;19(2-3):271-85</Citation><ArticleIdList><ArticleId IdType="pubmed">1367241</ArticleId></ArticleIdList></Reference><Reference><Citation>Biosci Biotechnol Biochem. 2003 Jan;67(1):1-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12619666</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1994 Dec;60(12):4387-93</Citation><ArticleIdList><ArticleId IdType="pubmed">7811079</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 1997 Apr;21(2):214-22</Citation><ArticleIdList><ArticleId IdType="pubmed">9228789</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Microbiol. 2003 Oct;180(4):251-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12920506</ArticleId></ArticleIdList></Reference><Reference><Citation>Electrophoresis. 2002 Feb;23(4):655-61</Citation><ArticleIdList><ArticleId IdType="pubmed">11870778</ArticleId></ArticleIdList></Reference><Reference><Citation>World J Microbiol Biotechnol. 1990 Jun;6(2):209-17</Citation><ArticleIdList><ArticleId IdType="pubmed">24429995</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Biochem. 1975 Feb 3;51(1):213-8</Citation><ArticleIdList><ArticleId IdType="pubmed">235428</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1992 Aug;174(15):5036-42</Citation><ArticleIdList><ArticleId IdType="pubmed">1629160</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Biochem. 1975 Nov 15;59(2):607-13</Citation><ArticleIdList><ArticleId IdType="pubmed">1225521</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1985 Jul 15;260(14):8348-53</Citation><ArticleIdList><ArticleId IdType="pubmed">2989288</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1991 May;57(5):1453-60</Citation><ArticleIdList><ArticleId IdType="pubmed">1854201</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2001 Nov 23;276(47):43958-69</Citation><ArticleIdList><ArticleId IdType="pubmed">11551941</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 1999 Apr 1;339 ( Pt 1):43-53</Citation><ArticleIdList><ArticleId IdType="pubmed">10085226</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 1995 Dec 15;43(3):183-94</Citation><ArticleIdList><ArticleId IdType="pubmed">8590644</ArticleId></ArticleIdList></Reference><Reference><Citation>Electrophoresis. 2002 Jul;23(14):2184-93</Citation><ArticleIdList><ArticleId IdType="pubmed">12210222</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1985 Mar 10;260(5):2609-12</Citation><ArticleIdList><ArticleId IdType="pubmed">2982828</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 2000 Mar 10;78(2):93-113</Citation><ArticleIdList><ArticleId IdType="pubmed">10725534</ArticleId></ArticleIdList></Reference><Reference><Citation>Met Ions Biol Syst. 2000;37:559-86</Citation><ArticleIdList><ArticleId IdType="pubmed">10693145</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1993 Feb 1;300(2):705-13</Citation><ArticleIdList><ArticleId IdType="pubmed">8434950</ArticleId></ArticleIdList></Reference><Reference><Citation>Yeast. 1998 Jan 15;14(1):67-76</Citation><ArticleIdList><ArticleId IdType="pubmed">9483796</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 1987;41:465-505</Citation><ArticleIdList><ArticleId IdType="pubmed">3318677</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation><ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1989 Dec 5;264(34):20350-5</Citation><ArticleIdList><ArticleId IdType="pubmed">2584220</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><noCountry><name sortKey="Koc, Hasan" sort="Koc, Hasan" uniqKey="Koc H" first="Hasan" last="Koc">Hasan Koc</name><name sortKey="Liu, Feng" sort="Liu, Feng" uniqKey="Liu F" first="Feng" last="Liu">Feng Liu</name><name sortKey="Tien, Ming" sort="Tien, Ming" uniqKey="Tien M" first="Ming" last="Tien">Ming Tien</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Abbas, Ahmed" sort="Abbas, Ahmed" uniqKey="Abbas A" first="Ahmed" last="Abbas">Ahmed Abbas</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000832 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000832 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:15551134
|texte= Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15551134" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |